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Cancer Primary Screening

DNA Methylation Detection Kit for Human PCDH17, POU4F2 and PENK Gene -- BLAC (Bladder Cancer)

Clinical Application

Bladder cancer is one of the most common malignancies but the corresponding diagnostic methods are either invasive or limited in specificity and/or sensitivity. DNA methylation is present in all bladder tumors and can be detected in urine specimens.

DNA Methylation can be a novel marker of prognosis for tumor therapy, which is also a reversible process in which inactivated tumor suppressor genes are expressed through demethylation to exert a tumor suppressor effect. Therefore, demethylation can be used as a new direction in cancer treatment to monitor the dynamics of the disease.

Intended Use

This kit is a real-time PCR assay for qualitative detection of DNA methylation in gene PCDH17, POU4F2 and PENK in human genomic DNA extracted from stool sample.

General Specification

Methodology: Real-Time PCR, PCR-Fluorescence Probing
Sample Type: urine
Analytical Sensitivity: 85.8%
Analytical Specificity: 90.6%
Precision: CV <5%
LoD: 1% of PCDH17, POU4F2 and PENK methylation in the background of 5ng human genomic DNA

Packing Specifications

24 tests/kit (non-single test/tube) 48 tests/kit (non-single test/tube)
 
                     

Work Flow



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